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Relative mRNA expression of gonadotropin-releasing hormone <t>(GnRH)</t> ( A , C ) (mean ± SEM) and GnRH protein levels ( B , D ) (mean ± SEM) in the mediobasal hypothalamus (MBH; A , B ) and preoptic area (POA; C , D ) of sheep treated with vehicle (C), a lower dose of microplastics (LD), or a higher dose of microplastics (HD). Significance of differences: * p < 0.05, *** p < 0.001.
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Relative mRNA expression of gonadotropin-releasing hormone <t>(GnRH)</t> ( A , C ) (mean ± SEM) and GnRH protein levels ( B , D ) (mean ± SEM) in the mediobasal hypothalamus (MBH; A , B ) and preoptic area (POA; C , D ) of sheep treated with vehicle (C), a lower dose of microplastics (LD), or a higher dose of microplastics (HD). Significance of differences: * p < 0.05, *** p < 0.001.
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Relative mRNA expression of gonadotropin-releasing hormone <t>(GnRH)</t> ( A , C ) (mean ± SEM) and GnRH protein levels ( B , D ) (mean ± SEM) in the mediobasal hypothalamus (MBH; A , B ) and preoptic area (POA; C , D ) of sheep treated with vehicle (C), a lower dose of microplastics (LD), or a higher dose of microplastics (HD). Significance of differences: * p < 0.05, *** p < 0.001.
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Relative mRNA expression of gonadotropin-releasing hormone (GnRH) ( A , C ) (mean ± SEM) and GnRH protein levels ( B , D ) (mean ± SEM) in the mediobasal hypothalamus (MBH; A , B ) and preoptic area (POA; C , D ) of sheep treated with vehicle (C), a lower dose of microplastics (LD), or a higher dose of microplastics (HD). Significance of differences: * p < 0.05, *** p < 0.001.

Journal: International Journal of Molecular Sciences

Article Title: Effects of Microplastics on the Central Reproductive Neuroendocrine System in a Sheep Model

doi: 10.3390/ijms27052316

Figure Lengend Snippet: Relative mRNA expression of gonadotropin-releasing hormone (GnRH) ( A , C ) (mean ± SEM) and GnRH protein levels ( B , D ) (mean ± SEM) in the mediobasal hypothalamus (MBH; A , B ) and preoptic area (POA; C , D ) of sheep treated with vehicle (C), a lower dose of microplastics (LD), or a higher dose of microplastics (HD). Significance of differences: * p < 0.05, *** p < 0.001.

Article Snippet: The GnRH concentration in the homogenates was determined using the Goat gonadotropin-releasing hormone, GnRH ELISA Kit (CSB-E13276G, Cusabio, Wuhan, China) according to the manufacturer’s protocol.

Techniques: Expressing